Part:BBa_K801000:Design

pTUM100 yeast shuttle vector based on pYES2

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found at 36
  Illegal suffix found at 114
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  Illegal NheI site found at 3379
  Illegal SpeI site found at 115
  Illegal PstI site found at 129
  Illegal NotI site found at 42
  Illegal NotI site found at 122
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 36
  Illegal XhoI site found at 63
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 36
  Illegal suffix found at 115
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 36
  Illegal suffix found at 105
  Illegal XbaI site found at 51
  Illegal NgoMIV site found at 69
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 79
  Illegal BsaI.rc site found at 1536
  Illegal BsaI.rc site found at 2482
  Illegal SapI site found at 453
  Illegal SapI site found at 2634
  

Keywords:

yeast shuttle vector, yeast, yeast vector, pTUM, pYES2

Abbreviations:

Design Notes

Related BioBrick:

Other versions:

• BBa_K801001: pTUM101 yeast shuttle vector with pTEF1 promoter
• BBa_K801002: pTUM102 yeast shuttle vector with pTEF2 promoter
• BBa_K801003: pTUM103 yeast shuttle vector with pADH1 promoter
• BBa_K801004: pTUM104 yeast shuttle vector with GAL1 promoter

Quality control measures:

• Part was totally sequenced

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Commercial system: pYES2 , Invitrogen

Organism:

References

Literature references:

• http://www.ncbi.nlm.nih.gov/pubmed/6392852 West et al., 1984 West, R. W. J., Yocum, R. R., and Ptashne, M. (1984). Saccharomyces cerevisiae GAL1-GAL10 Divergent Promoter Region: Location and Function of the Upstream Activator Sequence UASG. Mol. Cell. Biol.4, 2467-2478.
• http://www.ncbi.nlm.nih.gov/pubmed/3886158 Giniger et al., 1985 Giniger, E., Barnum, S. M., and Ptashne, M. (1985). Specific DNA Binding of GAL4, a Positive Regulatory Protein of Yeast. Cell 40, 767-774.

Database references: